ИСТИНА

A variety of biomaterials are widely used in modern regenerative medicine. Particularly in traumatology, products made from chemically stabilized bone matrices are used. Throughout the entire life of the implant, long-term protection against bacterial infection is required. Unlike living bone, a bioprosthesis is not able to resist bacterial attack on its own. The present work is devoted to the creation of new biocomposite materials based on bovine bone matrix covered with polysaccharide gel layers capable of locally releasing antibiotics when bacteria attack the prosthesis. A biocomposite material based on a bone matrix was obtained as follows: pre-washed bovine bone was treated with hydroxyethyl starch modified with epichlorohydrin with a covalently bound broad-spectrum antibiotic - amikacin. Comparative tests of the bacteriostatic effect of the gel layer were carried out using the method of inhibition of the growth of the culture of Staphylococcus aureus P209 on agar medium. When the bioprosthesis comes into contact with the staphylococcus lawn a zone of growth inhibition is observed in a day around the sample, under the samples and on their surface. Thus, the release of amikacin occurs locally under the influence of a bacterial attack and stops with the destruction and removal of bacteria. Thus, polymer systems for targeted delivery and controlled release of drugs have been developed, which allow the release of drugs from the prosthesis during a bacterial attack.