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Interaction in vivo between the two matrix attachment regions flanking a single chromatin loopстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Eivazova E.R., Gavrilov A., Pirozhkova I., Petrov A., Iarovaia O.V., Razin S.V., Lipinski M., Vassetzky Y.S.
- Журнал: Journal of Molecular Biology
- Том: 386
- Номер: 4
- Год издания: 2009
- Издательство: Academic Press
- Местоположение издательства: United States
- Первая страница: 929
- Последняя страница: 937
- DOI: 10.1016/j.jmb.2008.12.022
- Аннотация: In interphase nuclei as in metaphase chromosomes, the genome is organized into topologically closed loop domains. Here, we have mapped the ends of the loop domain that contains the Ifng (interferon-gamma) gene in primary and cultured murine T-lymphocytes. To determine whether the ends of the loop are located in close proximity to each other in the nuclear space, the 3C (chromosome conformation capture) technique, which detects protein-mediated DNA-DNA interactions, was utilized. A strong interaction was demonstrated between the two ends of the loop, which were close enough to become cross-linked in vivo in the presence of paraformaldehyde. Chromatin immunoprecipitation combined with the 3C technique demonstrated that topoisomerase IIalpha and MeCP2, but not topoisomerase IIbeta, heterochromatin-associated protein HP1 or CTCF, were involved in this interaction. The present findings have important implications in terms of mechanisms of illegitimate recombination that can result in chromosomal translocations and deletions.
- Добавил в систему: Разин Сергей Владимирович