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Effects of charges and dipole moments of membrane lipids on gating properties of ion channels induced by syringomycin Eтезисы доклада
- Авторы: Gurnev P.A., Kaulin Y.A., Takemoto J.Y., Schagina L.V., Malev V.V.
- Сборник: Biophysical Journal
- Том: 19
- Тезисы
- Год издания: 2002
- Первая страница: 243
- Последняя страница: 249
- Аннотация: Effects of surface charges and dipole potential of planar lipid bilayers on voltage sensitivity of ion channels induced by antibiotic syringomycin E (SRE) were studied. In negatively charged bilayers (DOPS/DOPE, 1 : 1, M:M) bathed in diluted 0.1 M NaCl solution (pH 6), the channels were opened at positive voltages (V) (with respect to the side of SRE addition) and closed, at negative ones. Screening of SIZE and lipid charges by increasing of NaCl concentration of the membrane bathing solution up to 1.0 M reverses the channel voltage dependence: channels are opened at V < 0 and closed at V > 0. The same voltage dependence of the channel opening/closure was observed in membranes formed by uncharged lipids (DPhPC) bathed in NaCl solutions (0.1-1.0 M, pH 6). These findings suggest participation of lipid charges in the SRE-channel gating and an essential contribution of dipole moments of lipid molecules in the opening/closure work of SRE channels. This suggestion is confirmed by estimations of the dipole constituent of the SRE-channel formation work and by experimental results obtained with phloretin-modified membranes.
- Добавил в систему: Каулин Юрий Альфредович