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Assay variables and early clinical evaluation of low-angle light scattering for platelet function analysisстатья Исследовательская статья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 декабря 2024 г.
- Авторы: Svidelskaya Galina S., Sorkina Vera P., Ignatova Anastasia A., Ponomarenko Evgeniya A., Poletaev Aleksandr V., Seregina Elena A., Manuvera Valentin A., Zharkov Pavel A., Mindukshev Igor V., Gambaryan Stepan, Panteleev Mikhail A.
- Журнал: International Journal of Hematology
- Том: 120
- Номер: 6
- Год издания: 2024
- Издательство: Japanese Society of Hematology
- Местоположение издательства: Japan
- Первая страница: 717
- Последняя страница: 724
- DOI: 10.1007/s12185-024-03859-0
- Аннотация: Introduction: The recently developed platelet aggregation technique based on low-angle light scattering (LaSca) in diluted platelet-rich plasma (PRP) requires only a small sample volume and provides information about platelet aggregation and shape change. This study aimed to investigate the influence of preanalytical and analytical variables and to validate the method in a real-life pediatric hematology hospital setting.Methods: Platelet aggregation was induced by ADP in diluted PRP in the presence of 2 mM calcium at 23 °C. The study included healthy adults (n = 30), healthy children (n = 20), and pediatric patients with suspected or diagnosed platelet function abnormalities (n = 25).Results: The assay parameters were stable for at least 3 h after isolation of PRP and were sensitive to plasma dilution in the range of 2-8%. The initial aggregation velocity was significantly reduced in pediatric patients compared with healthy children (p < 0.05). ADP-induced light transmission amplitude was moderately correlated with LaSca amplitude of aggregation in healthy children (p = 0.52, p < 0.05) but not in pediatric patients.Conclusions: We standardized the protocol for platelet aggregation assessment by LaSca and characterized the influence of preanalytical and analytical variables on it.
- Добавил в систему: Свидельская Галина Сергеевна