Аннотация:The increasing interest in the so-called rare genera of actinomycetes is due to the fact that theirpotential as producers of valuable metabolites, primarily antibiotic, has been studied insufficiently. The use of selective methods made it possible to isolate the YrIIa5 strain from the soil collected near the Dutch Fortress (Rostov, Yaroslavl oblast). Phylogenetic analysis based on the similarity of the 16S rRNA genes and five housekeeping genes (MLSA) allowed us to assign the isolate to the genus Krasilnikoviella. Genome analysis revealed many genes responsible for resistance to a wide range of antibiotics, which was confirmed by studying the strain for sensitivity to antimicrobial compounds using the agar diffusion method.