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Lecture Notes on History of Determination of Bio-Antioxidants and Neuromediators by GC- and GC-MS Techniques in Personalized Molecular Medicineстатья
- Авторы: Adamovic E.D., Gradov O.V., Orekhov F.K.
- Журнал: European Journal of Molecular Biotechnology
- Том: 12
- Номер: 1
- Год издания: 2024
- Первая страница: 33
- Последняя страница: 55
- DOI: 10.13187/ejmb.2024.1.33
- Аннотация: A significant task related to the practice of using various antioxidants in medicine is their detection in natural samples, pharmaceutical substances and their precursors, in human tissues and physiological secretions. Without registering the redox status of the latter, the transition from redox biology to redox medicine cannot be made. Without objective registration of redox status, a constructive approach to redox pathology, redox etiology and redox prevention of diseases by introducing antioxidant-containing foods and active additives into the diet is impossible. Therefore, technologies for analytical and analytical biochemistry of antioxidants are extremely important in setting problems in redox medicine. At the same time, many methods that are used for these purposes determine the activity, but not specific, selective in composition and reactivity in a given environment, chemical systems, an example of which is the potentiometric determination of redox-active forms. If we accept that many antioxidant schemes work effectively in micro-heterogeneous/ultramicroheterogeneous environments, and biological tissue with organelles with different electrophysical parameters is one of the most typical examples of these environments, then it becomes obvious that additive analysis, as well as assessment of the overall redox efficiencies are not stringent enough for redox medicine. It is necessary, at a minimum, to separately evaluate the concentrations of different redox components and antioxidants, and, as a maximum, to carry out position-sensitive analysis-in the compartments in which the reaction process takes place. The second task relates to typical cytochemistry or ultrastructural biochemistry, therefore it is not considered in this review, and the first task is simple, from the point of view of measurements at individual points or with averaging over a sample-that is, standard methods of bioanalytical chemistry. In particular, methods of gas chromatography of antioxidants, which are most intensively developed in the aspect of GC-MS, can be considered the optimal means for achieving such analytical goals. However, classical gas chromatographic techniques can also be used for these purposes, as will be shown in this historical bibliographic review, based on a course of lectures on these methods that has been formed since the 2000s to 2020s (due to which the depth of the search, unlike the latest reviews, is not limited to the last few years, and the review has a significant historical bias). Accepted: May 17, 2024.
- Добавил в систему: Gradov Oleg V.